4.7 Article

Exosomal miR-122 promotes adipogenesis and aggravates obesity through the VDR/SREBF1 axis

期刊

OBESITY
卷 30, 期 3, 页码 666-679

出版社

WILEY
DOI: 10.1002/oby.23365

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资金

  1. Major Science and Technology Program of Hainan Province [ZDKJ2019010]
  2. High-level Talents Project of Hainan Basic and Applied Basic Research Program (Natural Science) [2019RC390]

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This study found that miR-122-enriched exosomes promote adipogenesis by regulating the VDR/SREBF1 axis. Inhibition of miR-122 can alleviate obesity.
Objective: This study examined the effects of miR-122-enriched exosomes on the expression of vitamin D3 receptor (VDR) and sterol regulatory element-binding transcription factor 1 (SREBF1) and their roles during adipogenesis. Methods: The roles of miR-122, SREBF1, and VDR were investigated during adipogenesis. The relationships between VDR and miR-122 or SREBF1 were assessed by dual-luciferase reporter and chromatin immunoprecipitation assays. The potential role of miR-122/VDR/SREBF1 was evaluated in high-fat diet-induced obese male mice. Results: High levels of miR-122 were found only in adipose tissue-derived exosomes (Exo-AT) and Exo-AT-treated cells. Overexpression of miR-122 promoted adipogenesis, and inhibition of miR-122 prevented adipogenesis by regulating VDR, SREBF1, peroxisome proliferator-activated receptor gamma, lipoprotein lipase, and adiponectin. Knockdown of Srebf1 or overexpression of VDR could inhibit adipogenesis. However, exosomal miR-122 could reverse their inhibitory effects. The dual-luciferase reporter assay and chromatin immunoprecipitation assays confirmed that VDR was a direct target of miR-122. It could bind to the BS1 region of the SREBF1 promoter and inhibit SREBF1 expression. Moreover, miR-122 inhibition could alleviate obesity in high-fat diet-induced obese male mice, possibly through upregulating the VDR/SREBF1 axis. Conclusion: MiR-122-enriched Exo-AT promoted adipogenesis by regulating the VDR/SREBF1 axis.

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