期刊
NUCLEIC ACIDS RESEARCH
卷 50, 期 4, 页码 1864-1874出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkac097
关键词
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资金
- Intramural Research Program of the National Library of Medicine at the U.S. National Institutes of Health
- Ontario Institute of Cancer Research
- Natural Sciences and Engineering Research Council of Canada
- Canada Research Chairs
This study investigates the effects of cytosine methylation at CpG sites on nucleosome dynamics and stability. The results show that methylation induces significant changes in the geometry of DNA, leading to enhanced interactions with the histone octamer and preventing DNA unwrapping.
Cytosine methylation at the 5-carbon position is an essential DNA epigenetic mark in many eukaryotic organisms. Although countless structural and functional studies of cytosine methylation have been reported, our understanding of how it influences the nucleosome assembly, structure, and dynamics remains obscure. Here, we investigate the effects of cytosine methylation at CpG sites on nucleosome dynamics and stability. By applying long molecular dynamics simulations on several microsecond time scale, we generate extensive atomistic conformational ensembles of full nucleosomes. Our results reveal that methylation induces pronounced changes in geometry for both linker and nucleosomal DNA, leading to a more curved, under-twisted DNA, narrowing the adjacent minor grooves, and shifting the population equilibrium of sugar-phosphate backbone geometry. These DNA conformational changes are associated with a considerable enhancement of interactions between methylated DNA and the histone octamer, doubling the number of contacts at some key arginines. H2A and H3 tails play important roles in these interactions, especially for DNA methylated nucleosomes. This, in turn, prevents a spontaneous DNA unwrapping of 3-4 helical turns for the methylated nucleosome with truncated histone tails, otherwise observed in the unmethylated system on several microseconds time scale.
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