期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 21, 页码 12517-12534出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab1098
关键词
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资金
- National Research Foundation (NRF) of Korea - Korean government (Ministry of Science, ICT and Future Planning) [NRF-2015R1A3A2033665, NRF-2018R1A5A1024261]
- Korea University Grant
- NRF - Korea government (Ministry of Science, ICT and Future Planning) [NRF-2021R1C1C2013476]
This study identified a surveillance pathway in which pioneer translation ensures proper targeting of endoplasmic reticulum and mitochondrial proteins, preventing abnormal protein folding and cytosolic stress response.
The pioneer (or first) round of translation of newly synthesized mRNAs is largely mediated by a nuclear cap-binding complex (CBC). In a transcriptome-wide analysis of polysome-associated and CBC-bound transcripts, we identify RN7SL1, a noncoding RNA component of a signal recognition particle (SRP), as an interaction partner of the CBC. The direct CBC-SRP interaction safeguards against abnormal expression of polypeptides from a ribosome-nascent chain complex (RNC)-SRP complex until the latter is properly delivered to the endoplasmic reticulum. Failure of this surveillance causes abnormal expression of misfolded proteins at inappropriate intracellular locations, leading to a cytosolic stress response. This surveillance pathway also blocks protein synthesis through RNC-SRP misassembled on an mRNA encoding a mitochondrial protein. Thus, our results reveal a surveillance pathway in which pioneer translation ensures proper targeting of endoplasmic reticulum and mitochondrial proteins.
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