4.8 Article

Genome-wide chromosomal association of Upf1 is linked to Pol II transcription in Schizosaccharomyces pombe

期刊

NUCLEIC ACIDS RESEARCH
卷 50, 期 1, 页码 350-367

出版社

OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab1249

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资金

  1. Wellcome Trust [9340/Z/09/Z, 202115/Z/16/Z]
  2. Royal Society [RG170246]
  3. DBT Ramalingaswami Grant [BT/RHD/35/02/2006]
  4. Biotechnology and Biological Sciences Research Council [BBSRC BB/M022757/1, BBSRC BB/S017984/1]
  5. Bangabandhu Science and Technology Fellowship Trust
  6. Wellcome Trust [202115/Z/16/Z] Funding Source: Wellcome Trust

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The study found that the RNA helicase Upf1 in Schizosaccharomyces pombe is associated with active genes genome-wide, correlates with Pol II transcription and mRNA expression levels, and may regulate gene expression by influencing Pol II phosphorylation and transcription.
Although the RNA helicase Upf1 has hitherto been examined mostly in relation to its cytoplasmic role in nonsense mediated mRNA decay (NMD), here we report high-throughput ChIP data indicating genome-wide association of Upf1 with active genes in Schizosaccharomyces pombe. This association is RNase sensitive, correlates with Pol II transcription and mRNA expression levels. Changes in Pol II occupancy were detected in a Upf1 deficient (upf1 Delta) strain, prevalently at genes showing a high Upf1 relative to Pol II association in wild-type. Additionally, an increased Ser2 Pol II signal was detected at all highly transcribed genes examined by ChIP-qPCR. Furthermore, upf1 Delta cells are hypersensitive to the transcription elongation inhibitor 6-azauracil. A significant proportion of the genes associated with Upf1 in wild-type conditions are also mis-regulated in upf1 Delta. These data envisage that by operating on the nascent transcript, Upf1 might influence Pol II phosphorylation and transcription.

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