期刊
NUCLEIC ACIDS RESEARCH
卷 49, 期 20, 页码 11959-11973出版社
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab963
关键词
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资金
- Canadian Institutes of Health Research (CIHR) [PJT148801]
- Bristol-Myers-Squibb Research Chair in Molecular Biology at the Universite de Montreal
- Fonds de Recherche du Quebec - Nature et Technologies (FRQ-NT)
- Universite de Montreal (UdeM)
- FRQ-NT
- NIH/NIGMS [DMR1829070, GM-124166]
- Canada Foundation for Innovation [30574]
- Canadian Institutes ofHealthResearch (CIHR) [PJT-148801]
- Luigi-Liberatore Foundation
- UdeM
- NSF
The integrative approach combining NMR and SAXS studies using the divide-and-conquer strategy successfully determined the solution structure of the Neurospora VS ribozyme. Through a multi-step procedure, refined NMR structures were paired with SAXS data to assemble subdomain structures, which were then filtered to reveal a global conformational change associated with substrate binding. This study highlights the importance of similar integrative approaches for structural studies of other large RNAs.
The divide-and-conquer strategy is commonly used for protein structure determination, but its applications to high-resolution structure determination of RNAs have been limited. Here, we introduce an integrative approach based on the divide-and-conquer strategy that was undertaken to determine the solution structure of an RNA model system, the Neurospora VS ribozyme. NMR and SAXS studies were conducted on a minimal trans VS ribozyme as well as several isolated subdomains. A multi-step procedure was used for structure determination that first involved pairing refined NMR structures with SAXS data to obtain structural subensembles of the various subdomains. These subdomain structures were then assembled to build a large set of structural models of the ribozyme, which was subsequently filtered using SAXS data. The resulting NMR-SAXS structural ensemble shares several similarities with the reported crystal structures of the VS ribozyme. However, a local structural difference is observed that affects the global fold by shifting the relative orientation of the two three-way junctions. Thus, this finding highlights a global conformational change associated with substrate binding in the VS ribozyme that is likely critical for its enzymatic activity. Structural studies of other large RNAs should benefit from similar integrative approaches that allow conformational sampling of assembled fragments.
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