Chromosome-specific painting unveils chromosomal fusions and distinct allopolyploid species in the Saccharum complex

Karyotypes provide key cytogenetic information on the phylogenetic relationships and evolutionary origins in related eukaryotic species. Despite our knowledge of the chromosome numbers of sugarcane and its wild relatives, the chromosome composition and evolution among the species in the Saccharum complex have been elusive owing to the complex polyploidy and the large numbers of chromosomes of these species. Oligonucleotide-based chromosome painting has become a powerful tool of cytogenetic studies especially for plant species with large numbers of chromosomes. We developed oligo-based chromosome painting probes for all 10 chromosomes in Saccharum officinarum (2n = 8x = 80). The 10 painting probes generated robust fluorescence in situ hybridization signals in all plant species within the Saccharum complex, including species in the genera Saccharum, Miscanthus, Narenga and Erianthus. We conducted comparative chromosome analysis using the same set of probes among species from four different genera within the Saccharum complex. Excitingly, we discovered several novel cytotypes and chromosome rearrangements in these species. We discovered that fusion from two different chromosomes is a common type of chromosome rearrangement associated with the species in the Saccharum complex. Such fusion events changed the basic chromosome number and resulted in distinct allopolyploids in the Saccharum complex.

Automated summary

Karyotypes provide important cytogenetic information for understanding phylogenetic relationships and evolutionary origins in related eukaryotic species. Oligonucleotide-based chromosome painting has emerged as a powerful tool for cytogenetic studies, especially in plant species with a large number of chromosomes. By developing oligo-based chromosome painting probes for Saccharum officinarum and conducting comparative chromosome analysis among different species within the Saccharum complex, novel cytotypes and chromosome rearrangements were discovered, shedding light on the evolution of these species.