期刊
NEW PHYTOLOGIST
卷 233, 期 4, 页码 1750-1767出版社
WILEY
DOI: 10.1111/nph.17898
关键词
arabinogalactan-protein (AGP); cell wall integrity (CWI); cellulose; Fasciclin-Like AGP (FLA); lignin; mechanical stress; secondary cell wall (SCW)
资金
- La Trobe University BioImaging platform
- University of Melbourne Research Scholarship
- Albert Shimmins Fund
- Australia Research Council [CE1101007]
- La Trobe Research Focus Area grant [2000004372]
- La Trobe University
- Zhejiang AF University
- Australian Research Council [CE1101007] Funding Source: Australian Research Council
This study suggests that FLA11 and FLA12 may act as cell surface sensors in plant xylem cells, allowing them to sense mechanical stimuli and regulate secondary cell wall (SCW) development. Overexpression of FLA11 promotes earlier SCW development, while overexpression of FLA12 increases cellulose content. These proteins play a role in modulating SCW responses to mechanical stress.
Secondary cell walls (SCWs) in stem xylem vessel and fibre cells enable plants to withstand the enormous compressive forces associated with upright growth. It remains unclear if xylem vessel and fibre cells can directly sense mechanical stimuli and modify their SCW during development. We provide evidence that Arabidopsis SCW-specific Fasciclin-Like Arabinogalactan-proteins 11 (FLA11) and 12 (FLA12) are possible cell surface sensors regulating SCW development in response to mechanical stimuli. Plants overexpressing FLA11 (OE-FLA11) showed earlier SCW development compared to the wild-type (WT) and altered SCW properties that phenocopy WT plants under compression stress. By contrast, OE-FLA12 stems showed higher cellulose content compared to WT plants, similar to plants experiencing tensile stress. fla11, OE-FLA11, fla12, and OE-FLA12 plants showed altered SCW responses to mechanical stress compared to the WT. Quantitative polymerase chain reaction (qPCR) and RNA-seq analysis revealed the up-regulation of genes and pathways involved in stress responses and SCW synthesis and regulation. Analysis of OE-FLA11 nst1 nst3 plants suggests that FLA11 regulation of SCWs is reliant on classical transcriptional networks. Our data support the involvement of FLA11 and FLA12 in SCW sensing complexes to fine-tune both the initiation of SCW development and the balance of lignin and cellulose synthesis/deposition in SCWs during development and in response to mechanical stimuli.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据