期刊
NEW PHYTOLOGIST
卷 235, 期 2, 页码 718-731出版社
WILEY
DOI: 10.1111/nph.17972
关键词
basic helix-loop-helix; bryophytes; moss; Physcomitrium patens; sporangium; sporogenesis; tapetum; transcription factor
资金
- Swedish Research Council [621-2014-4941, 2018-04068]
- Nilsson-Ehle Endowments [41629]
- Swedish Research Council [2018-04068] Funding Source: Swedish Research Council
This study investigates the conservation of regulation of sporogenous cells and nursing cells surrounding them between angiosperm anthers and bryophyte sporangia. It demonstrates that the Clade II bHLH TFs play similar roles in the development of spore precursors in Physcomitrium patens and angiosperms, supporting the hypothesis of their relatedness.
Clade II basic helix-loop-helix transcription factors (bHLH TFs) are essential for pollen production and tapetal nursing functions in angiosperm anthers. As pollen has been suggested to be related to bryophyte spores by descent, we characterized two Physcomitrium (Physcomitrella) patens clade II bHLH TFs (PpbHLH092 and PpbHLH098), to test if regulation of sporogenous cells and the nursing cells surrounding them is conserved between angiosperm anthers and bryophyte sporangia. We made CRISPR-Cas9 reporter and loss-of-function lines to address the function of PpbHLH092/098. We sectioned and analyzed WT and mutant sporophytes for a comprehensive stage-by-stage comparison of sporangium development. Spore precursors in the P. patens sporangium are surrounded by nursing cells showing striking similarities to tapetal cells in angiosperms. Moss clade II bHLH TFs are essential for the differentiation of these tapetal-like cells and for the production of functional spores. Clade II bHLH TFs provide a conserved role in controlling the sporophytic somatic cells surrounding and nursing the sporogenous cells in both moss sporangia and angiosperm anthers. This supports the hypothesis that such nursing functions in mosses and angiosperms, lineages separated by roughly 450 million years, are related by descent.
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