期刊
NEW PHYTOLOGIST
卷 233, 期 6, 页码 2471-2487出版社
WILEY
DOI: 10.1111/nph.17812
关键词
ALMT1; aluminium; Arabidopsis roots; CML24; malate exudation
资金
- National Natural Science Foundation of China [31670259, 31872655, 32070303, 31400227]
- Shandong Provincial Key Research and Development Program [2019GSF107005]
- Shandong Provincial Natural Science Foundation Project [ZR2014CQ021]
- Fundamental Research Funds of Shandong University [2018JC059]
The study reveals the crucial role of CML24 in Arabidopsis aluminum resistance by regulating ALMT1-mediated malate exudation, interacting with CAMTA2 to enhance transcriptional activity, and suppressing the transcriptional repression of ALMT1 by WRKY46.
ALUMINUM-ACTIVATED MALATE TRANSPORTER1 (ALMT1)-mediated malate exudation from roots is critical for aluminium (Al) resistance in Arabidopsis. Its upstream molecular signalling regulation is not yet well understood. The role of CALMODULIN-LIKE24 (CML24) in Al-inhibited root growth and downstream molecular regulation of ALMT1-meditaed Al resistance was investigated. CML24 confers Al resistance demonstrated by an increased root-growth inhibition of the cml24 loss-of-function mutant under Al stress. This occurs mainly through the regulation of the ALMT1-mediated malate exudation from roots. The mutation and overexpression of CML24 leads to an elevated and reduced Al accumulation in the cell wall of roots, respectively. Al stress induced both transcript and protein abundance of CML24 in root tips, especially in the transition zone. CML24 interacts with CALMODULIN BINDING TRANSCRIPTION ACTIVATOR2 (CAMTA2) and promotes its transcriptional activity in the regulation of ALMT1 expression. This results in an enhanced malate exudation from roots and less root-growth inhibition under Al stress. Both CML24 and CAMTA2 interacted with WRKY46 suppressing the transcriptional repression of ALMT1 by WRKY46. The study provides novel insights into understanding of the upstream molecular signalling of the ALMT1-depdendent Al resistance.
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