期刊
NEUROSCIENCE
卷 473, 期 -, 页码 130-141出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.neuroscience.2021.08.006
关键词
alpha-asarone; cerebral ischemia-reperfusion; stroke; glial activation; autophagy
资金
- National Natural Science Foundation of China [81473168]
- Key Research and Development Projects of Sichuan Department of Science and Technology, China [2019YFS0328]
The study found that alpha-asarone has a desirable neuroprotective effect against CIR stroke in rats, reducing infarct volume and post-stroke epilepsy, and improving neurological function. Further investigation revealed that alpha-asarone alleviated cell injury and apoptosis, improved glial activation and autophagy to attenuate CIR stroke injury.
Alpha-asarone, a major active component isolated from Acorus gramineus, can affect brain functions and behaviors by multiple mechanisms. However, the effect of alpha-asarone on cerebral ischemia-reperfusion (CIR) stroke has not been reported. The present study aimed to investigate the neuroprotective effect of alpha-asarone and the involved mechanisms against CIR stroke. Rats were subjected to middle cerebral occlusion (MCAO) for 2 h. Then the drug or drug-free vehicle was intravenously injected to corresponding groups. After reperfusion for 24 h, the infarct volume was evaluated by Triphenyl Tetrazolium Chloride (TTC) staining. The neurofunctional recovery and post-stroke epilepsy were evaluated. Nissl and Hematoxylin-Eosin (H&E) staining were used for histological observation. We investigated the protective mechanism of alpha-asarone against the stroke. The results showed that alpha-asarone exhibited a desirable neuroprotective effect, manifested as reducing infarct volume and post-stroke epilepsy and improving neurological function. Histological and flow cytometry analysis revealed that alpha-asarone treatment alleviated cell injury and apoptosis in vivo and in vitro. Further-more, alpha-asarone decreased GFAP, Iba-1, and LC3II/LC3I expression and increased the expression of p62. These results suggested that alpha-asarone attenuated the CIR stroke injury via ameliorating glial activation and autophagy. (C) 2021 IBRO. Published by Elsevier Ltd. All rights reserved.
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