4.2 Article

Forkhead Box 1(FoxO1) mediates psychological stress-induced neuroinflammation

期刊

NEUROLOGICAL RESEARCH
卷 44, 期 6, 页码 483-495

出版社

TAYLOR & FRANCIS LTD
DOI: 10.1080/01616412.2021.2022913

关键词

Neuroinflammation; epeated Social Defeat Stress (RSDS); Tumor Necrosis Factor-alpha (TNF-alpha); Interleukin 6 (IL-6); Interleukin-1 beta (IL-1 beta); Vascular Cell Adhesion Molecule-1 (VCAM-1)

资金

  1. National Natural Science Foundation of China [81871838]

向作者/读者索取更多资源

This study suggests that repeated social defeat stress (RSDS) can induce inflammation in both blood and brain, and FoxO1 may exacerbate stress-induced inflammation.
Objectives: Neuroinflammation plays a key role in cerebrovascular disease (CVD). Neuropsychiatric disorders appear to share an epidemiological association with inflammation, but the mechanisms are unclear. Forkhead box 1 (FoxO1) regulates inflammatory signaling in diabetes and cardiovascular diseases, but its role in psychological stress-induced neuroinflammation remains unknown. Therefore, we investigated the potential involvement of FoxO1 in repeated social defeat stress (RSDS)-induced neuroinflammation. Methods: 6-week-old male C57BL/6 J mice were randomly divided into RSDS or control groups. In the RSDS group, mice (18-22 g) were individually subjected to social defeat by an 8-week-old CD-1 mouse (28-32 g) for 10 min daily for 10 consecutive days. At 24 h after this 10-day process, corticosterone (CORD, epinephrine (EPI), hydrogen peroxide, and inflammatory factors (TNF-alpha, IL-6, IL-1 beta, and VCAM-1) from serum and brain tissues were assayed using ELISA, real-time PCR, and Western blot. Iba-1 was determined by immunofluorescence (IF), and FoxO1 siRNA was transfected into BV2 cells to further analyze the expression of inflammatory factors. Results: RSDS significantly increased the levels of TNF-alpha, IL-6, IL-1 beta, and VCAM-1 in the serum; it also increased both mRNA and protein expression of these in the brain. FoxO1 was significantly increased after stress, while its knockdown significantly suppressed stress-induced inflammation. Immunofluorescence demonstrated the activation of microglia in the setting of RSDS. Conclusion: RSDS induced a measurable inflammatory response in the blood and brain, and FoxO1 was demonstrated in vitro to aggravate stress-induced inflammation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.2
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据