Correlation of Cerebral and Subcutaneous Glycerol in Severe Traumatic Brain Injury and Association with Tissue Damage

Background This study is a substudy of a prospective consecutive double-blinded randomized study on the effect of prostacyclin in severe traumatic brain injury (sTBI). The aims of the present study were to investigate whether there was a correlation between brain and subcutaneous glycerol levels and whether the ratio of interstitial glycerol in the brain and subcutaneous tissue (glycerol(brain/sc)) was associated with tissue damage in the brain, measured by using the Rotterdam score, S-100B, neuron-specific enolase (NSE), the Injury Severity Score (ISS), the Acute Physiology and Chronic Health Evaluation Score (APACHE II), and trauma type. A potential association with clinical outcome was explored. Methods Patients with sTBI aged 15-70 years presenting with a Glasgow Coma Scale Score <= 8 were included. Brain and subcutaneous adipose tissue glycerol levels were measured through microdialysis in 48 patients, of whom 42 had complete data for analysis. Brain tissue damage was also evaluated by using the Rotterdam classification of brain computed tomography scans and the biochemical biomarkers S-100B and NSE. Results In 60% of the patients, a positive relationship in glycerol(brain/sc) was observed. Patients with a positive correlation of glycerol(brain/sc) had slightly higher brain glycerol levels compared with the group with a negative correlation. There was no significant association between the computed tomography Rotterdam score and glycerol(brain/sc). S-100B and NSE were associated with the profile of glycerol(brain/sc). Our results cannot be explained by the general severity of the trauma as measured by using the Injury Severity Score or Acute Physiology and Chronic Health Evaluation Score. Conclusions We have shown that peripheral glycerol may flux into the brain. This effect is associated with worse brain tissue damage. This flux complicates the interpretation of brain interstitial glycerol levels. We remind the clinicians that a damaged blood-brain barrier, as seen in sTBI, may alter the concentrations of various substances, including glycerol in the brain. Awareness of this is important in the interpretation of the data bedside as well in research.

Automated summary

This study investigated the correlation between brain and subcutaneous glycerol levels in sTBI patients, as well as the impact of this correlation on brain tissue damage. Results showed a positive relationship between glycerol(brain/sc) and S-100B and NSE levels, suggesting that damaged blood-brain barrier may alter concentrations of various substances in the brain. These findings emphasize the importance of considering the effects of blood-brain barrier integrity on data interpretation in clinical settings and research.