期刊
NEUROCHEMICAL RESEARCH
卷 47, 期 5, 页码 1369-1382出版社
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11064-022-03535-x
关键词
Oxymatrine; Hyperglycemia; Cerebral ischemia and reperfusion; Microvessel
资金
- National Natural Science Foundation of China [31960177, 31760280]
- Natural Science Foundation of Ningxia [2021AAC03152]
This study found that OMT may alleviate cerebral I/R injury under hyperglycemia by protecting microvessels. In both animal and in vitro cell experiments, OMT pre-treatment showed significant neuroprotective effects and promotion of angiogenesis.
Hyperglycemia aggravates cerebral ischemia/reperfusion (I/R) injury via vascular injury. There is still a lack of effective pharmaceutical preparations for cerebral I/R injury under hyperglycemia. This study aimed to investigate the effects of oxymatrine (OMT) on hyperglycemia-exacerbated cerebral I/R injury in vitro and in vivo. The middle cerebral artery occlusion (MCAO) and reperfusion was established in the rats under hyperglycemia. Meanwhile, oxygen-glucose deprivation and reoxygenation (OGD/R) with high glucose was used as an in vitro model of hyperglycemic cerebral I/R injury. The results showed that the neurological deficit score, mortality, infarct volume and penumbra apoptosis in hyperglycemia group were significantly higher than those in normal glucose group. OMT pre-treated obviously reduced the degree of neurological deficit, mortality, infarct volume, improve cerebral blood flow after I/R in rats with hyperglycemia, and increase the survival rate of human brain microvascular endothelial cells (HBMECs) in high glucose and OGD/R group. OMT significantly improved the ultrastructure changes of endothelial cells, and maintain the migration and angiogenesis potency of HBMECs in high glucose and OGD/R group. OMT obviously alleviated the down-regulating CD31 and CD105 expression in cerebral microvessels caused by hyperglycemia. It is concluded that OMT treatment might alleviate cerebral I/R injury under hyperglycemia via protecting microvessels.
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