期刊
NEURAL REGENERATION RESEARCH
卷 17, 期 7, 页码 1617-1622出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.330620
关键词
C2C12; denervation; ion channels; microRNA; miR-142a-3p; muscle; patch clamp; potassium; sodium; whole-cell currents
资金
- National Natural Science Foundation of China [82072162, 81971177]
- Beijing Municipal Natural Science Foundation of China [7192215]
This study demonstrates that changes in miR-142a-3p expression play a role in muscle cell electrophysiology by affecting ion channel currents.
MicroRNAs can regulate the function of ion channels in many organs. Based on our previous study we propose that miR-142a-39, which is highly expressed in denervated skeletal muscle, might affect cell excitability through similar mechanisms. In this study, we overexpressed or knocked down miR-142a-3p in C2C12 cells using a lentivirus method. After 7 days of differentiation culture, whole-cell currents were recorded. The results showed that overexpression of miR-142a-3p reduced the cell membrane capacitance, increased potassium current density and decreased calcium current density. Knockdown of miR-142a-3p reduced sodium ion channel current density. The results showed that change in miR-142a-3p expression affected the ion channel currents in C2C12 cells, suggesting its possible roles in muscle cell electrophysiology.
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