期刊
NEURAL REGENERATION RESEARCH
卷 17, 期 6, 页码 1357-1363出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.327353
关键词
alpha-synuclein; autophagosomes; autophagy; neural regeneration; neuroprotection; Parkinson's disease; PI3K/AKT/mTOR pathway; skin-derived precursor Schwann cells
资金
- Technology Project of Nantong of China [JC2020052, JCZ19087]
- National Natural Science Foundation of China [81873742, 81901195, 81502867, 82073627]
This study confirms that skin-derived precursor cells exhibit neuroprotective effects in Parkinson's disease by modulating the PI3K/AKT/mTOR pathway to inhibit autophagy.
Autophagy has been shown to play an important role in Parkinson's disease. We hypothesized that skin-derived precursor cells exhibit neuroprotective effects in Parkinson's disease through affecting autophagy. In this study, 6-hydroxydopamine-damaged SH-SYSY cells were pretreated with a culture medium containing skin-derived precursors differentiated into Schwann cells (SKP-SCs). The results showed that the SKP-SC culture medium remarkably enhanced the activity of SH-SYSY cells damaged by 6-hydroxydopamine, reduced excessive autophagy, increased tyrosine hydroxylase expression, reduced alpha-synuclein expression, reduced the autophagosome number, and activated the PI3K/AKT/mTOR pathway. Autophagy activator rapamycin inhibited the effects of SKP-SCs, and autophagy inhibitor 3-methyladenine had the opposite effect. These findings confirm that SKP-SCs modulate the PI3K/AKT/mTOR pathway to inhibit autophagy, thereby exhibiting a neuroprotective effect in a cellular model of Parkinson's disease. This study was approved by the Animal Ethics Committee of Laboratory Animal Center of Nantong University (approval No. 520181009-205) on October 9, 2018.
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