期刊
NEURAL REGENERATION RESEARCH
卷 17, 期 9, 页码 2043-2049出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.335160
关键词
crush injury; nerve injury; nerve regeneration; nitric oxide; peripheral nerve; revascularization; silica nanoparticles
资金
- National Research Foundation of Korea (NRF) - Ministry of Science and ICT [NRF2015R1C1A1A02036830, NRF-2015M3A9E2029186]
- Korea Institute of Science and Technology [2V05460/2V08630, 2E31121]
- Korea University Anam Hospital
This study demonstrates the use of NO-releasing silica nanoparticles to enhance revascularization and improve nerve regeneration in a rat model of peripheral nerve injury, suggesting a potential therapeutic strategy for promoting functional recovery after nerve damage.
Nitric oxide (NO) has been shown to promote revascularization and nerve regeneration after peripheral nerve injury. However, in vivo application of NO remains challenging due to the lack of stable carrier materials capable of storing large amounts of NO molecules and releasing them on a clinically meaningful time scale. Recently, a silica nanoparticle system capable of reversible NO storage and release at a controlled and sustained rate was introduced. In this study, NO-releasing silica nanoparticles (NO-SNs) were delivered to the peripheral nerves in rats after acute crush injury, mixed with natural hydrogel, to ensure the effective application of NO to the lesion. Microangiography using a polymer dye and immunohistochemical staining for the detection of CD34 (a marker for revascularization) results showed that NO-releasing silica nanoparticles increased revascularization at the crush site of the sciatic nerve. The sciatic functional index revealed that there was a significant improvement in sciatic nerve function in NO-treated animals. Histological and anatomical analyses showed that the number of myelinated axons in the crushed sciatic nerve and wet muscle weight excised from NO-treated rats were increased. Moreover, muscle function recovery was improved in rats treated with NO-SNs. Taken together, our results suggest that NO delivered to the injured sciatic nerve triggers enhanced revascularization at the lesion in the early phase after crushing injury, thereby promoting axonal regeneration and improving functional recovery.
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