期刊
NEURAL REGENERATION RESEARCH
卷 17, 期 2, 页码 386-394出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/1673-5374.314315
关键词
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资金
- National Natural Science Foundation of China [81471238, 81771327]
- Beijing Municipal Health Commission [PXM2020_026280_000002]
This study used tsRNA and mRNA transcriptome sequencing to investigate the expression changes in tsRNAs after traumatic brain injury in a mouse model. The results identified 103 differentially expressed tsRNAs, including 56 upregulated and 47 downregulated. Interaction pairs between differentially expressed tsRNAs and mRNAs were also identified. Gene Ontology analysis revealed enrichment in terms associated with inflammation and synaptic function.
Transfer RNA (tRNA)-derived small RNAs (tsRNAs) are a recently established family of regulatory small non-coding RNAs that modulate diverse biological processes. Growing evidence indicates that tsRNAs are involved in neurological disorders and play a role in the pathogenesis of neurodegenerative disease. However, whether tsRNAs are involved in traumatic brain injury-induced secondary injury remains poorly understood. In this study, a mouse controlled cortical impact model of traumatic brain injury was established, and integrated tsRNA and messenger RNA (mRNA) transcriptome sequencing were used. The results revealed that 103 tsRNAs were differentially expressed in the mouse model of traumatic brain injury at 72 hours, of which 56 tsRNAs were upregulated and 47 tsRNAs were downregulated. Based on microRNA-like seed matching and Pearson correlation analysis, 57 differentially expressed tsRNA-mRNA interaction pairs were identified, including 29 tsRNAs and 26 mRNAs. Moreover, Gene Ontology annotation of target genes revealed that the significantly enriched terms were primarily associated with inflammation and synaptic function. Collectively, our findings suggest that tsRNAs may be associated with traumatic brain injury-induced secondary brain injury, and are thus a potential therapeutic target for traumatic brain injury. The study was approved by the Beijing Neurosurgical Institute Animal Care and Use Committee (approval No. 20190411) on April 11, 2019.
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