期刊
NATURE METHODS
卷 19, 期 2, 页码 223-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41592-021-01378-y
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资金
- National Institutes of Health [1DP1DK113643]
- Paul G. Allen Family Foundation [0034665]
- Rutgers Cancer Institute of New Jersey New Investigator Award from the Cancer Center Support Grant [P30 CA072720]
Isotope imaging enables quantification of metabolic activity in mammalian tissues with spatial resolution, revealing metabolic heterogeneity in the kidney and brain and spatial gradients in metabolic processes.
Isotope tracing has helped to determine the metabolic activities of organs. Methods to probe metabolic heterogeneity within organs are less developed. We couple stable-isotope-labeled nutrient infusion to matrix-assisted laser desorption ionization imaging mass spectrometry (iso-imaging) to quantitate metabolic activity in mammalian tissues in a spatially resolved manner. In the kidney, we visualize gluconeogenic flux and glycolytic flux in the cortex and medulla, respectively. Tricarboxylic acid cycle substrate usage differs across kidney regions; glutamine and citrate are used preferentially in the cortex and fatty acids are used in the medulla. In the brain, we observe spatial gradations in carbon inputs to the tricarboxylic acid cycle and glutamate under a ketogenic diet. In a carbohydrate-rich diet, glucose predominates throughout but in a ketogenic diet, 3-hydroxybutyrate contributes most strongly in the hippocampus and least in the midbrain. Brain nitrogen sources also vary spatially; branched-chain amino acids contribute most in the midbrain, whereas ammonia contributes in the thalamus. Thus, iso-imaging can reveal the spatial organization of metabolic activity. Iso-imaging integrates stable-isotope infusions with imaging mass spectrometry to enable quantitative analysis of metabolic activity in mammalian tissues with spatial resolution. IsoScope software facilitates analysis of iso-imaging data.
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