期刊
NATURE IMMUNOLOGY
卷 23, 期 1, 页码 33-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41590-021-01088-9
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资金
- Foundation for the National Institutes of Health [T32 AI007290-35, R01 AR078268, U19 AI057229, U01 AI101981]
- National Science Foundation [DGE-1656518]
- German Research Foundation [LA3657/1]
This study investigated the B cell response to the BNT162b2 vaccine by integrating B cell repertoire analysis with single-cell transcriptomics pre- and post-vaccination. It found that the first vaccine dose generated a recall response of IgA(+) plasmablasts targeting the S subunit S2, while the second dose strongly boosted this response and produced potent neutralizing antibodies against SARS-CoV-2 and its variants.
The first ever US Food and Drug Administration-approved messenger RNA vaccines are highly protective against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)(1-3). However, the contribution of each dose to the generation of antibodies against SARS-CoV-2 spike (S) protein and the degree of protection against novel variants warrant further study. Here, we investigated the B cell response to the BNT162b2 vaccine by integrating B cell repertoire analysis with single-cell transcriptomics pre- and post-vaccination. The first vaccine dose elicits a recall response of IgA(+) plasmablasts targeting the S subunit S2. Three weeks after the first dose, we observed an influx of minimally mutated IgG(+) memory B cells that targeted the receptor binding domain on the S subunit S1 and likely developed from the naive B cell pool. This response was strongly boosted by the second dose and delivers potently neutralizing antibodies against SARS-CoV-2 and several of its variants.
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