期刊
INFECTION GENETICS AND EVOLUTION
卷 44, 期 -, 页码 130-136出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.meegid.2016.06.045
关键词
Subgroup J avian leukosis virus; Dendritic cell; Apoptosis; Cytokines; Immunosuppression
资金
- Key Project grant from Agricultural Ministry [CARS-42-G09]
- Modern Agriculture Talents Support Program [160]
- Public Industry Research Program of the Ministry of Agriculture, China [201203055]
- Natural Science Foundation of Guangdong Province [2014A030313450]
- Exceptional Talent Special Support Program of Guangzhou [E15237]
- Three High Agriculture Special Funds of Guangzhou [141]
Subgroup J avian leukosis virus (ALV-J) is an oncogenic retrovirus known to induce tumor formation and immunosuppression in infected chickens. One of the organs susceptible to ALV-J is the bone marrow, from which specialized antigen-presenting cells named dendritic cells (BM-DCs) are derived. Notably, these cells possess the unique ability to induce primary immune responses. In the present study, a method of cultivating and purifying DCs from chicken bone marrow in vitro was established to investigate the effects of ALV-J infection on BM-DC differentiation or generation. The results indicated that ALV-J not only infects the chicken bone marrow mononuclear cells but also appears to inhibit the differentiation and maturation of BM-DCs and to trigger apoptosis. Moreover, substantial reductions in the mRNA expression of TLR1, TLR2, TLR3, MHCI, and MHCII and in cytokine production were detected in the surviving BM-DCs following ALV-J infection. These findings indicate that ALV-J infection disrupts the process of bone marrow mononuclear cell differentiation into BM-DCs likely via altered antigen presentation, resulting in a downstream immune response in affected chickens. (C) 2016 Elsevier B.V. All rights reserved.
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