期刊
NATURE BIOTECHNOLOGY
卷 40, 期 3, 页码 335-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41587-021-01195-w
关键词
-
资金
- U.S. Department of Energy Bioenergy Technologies Office [DE-EE0007566]
- LanzaTech [CRADA/NFE-16-06364]
- Oak Ridge National Laboratory [CRADA/NFE-16-06364]
- U.S. Department of Energy Office of Science, Biological and Environmental Research Division, Genomic Science Program [DE-SC0018249, FWP ERKP903]
- U.S. Department of Energy [DE-AC05-00OR22725]
- Joint Genome Institute Community Science Program [CSP-503280]
- Office of Science of the U.S. Department of Energy [DE-AC02-05CH11231]
- National Defense Science and Engineering Graduate Fellowship [ND-CEN-017-095]
We have developed a carbon-negative fermentation route for producing acetone and isopropanol from waste gas, which fixes carbon and has a negative carbon footprint. By engineering acetogens, we achieved sustainable, high-efficiency, high-selectivity chemicals production.
Many industrial chemicals that are produced from fossil resources could be manufactured more sustainably through fermentation. Here we describe the development of a carbon-negative fermentation route to producing the industrially important chemicals acetone and isopropanol from abundant, low-cost waste gas feedstocks, such as industrial emissions and syngas. Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum. Next, we used omics analysis, kinetic modeling and cell-free prototyping to optimize flux. Finally, we scaled-up our optimized strains for continuous production at rates of up to similar to 3 g/L/h and similar to 90% selectivity. Life cycle analysis confirmed a negative carbon footprint for the products. Unlike traditional production processes, which result in release of greenhouse gases, our process fixes carbon. These results show that engineered acetogens enable sustainable, high-efficiency, high-selectivity chemicals production. We expect that our approach can be readily adapted to a wide range of commodity chemicals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据