Phytoconstituents from Adenanthera pavonina L. as antioxidants and inhibitors of inducible TNF-α production in BV2 cells

Adenanthera pavonina L has been used traditionally to relieve inflammation. This study aimed to expand the phytochemical knowledge of A. pavonina and evaluate its constituents for their antioxidant and anti-inflammatory potentials as tumor necrosis factor alpha (TNF-alpha) inhibitors. The antioxidant activity was evaluated using the DPPH radical scavenging assay, and the inhibition of TNF-alpha was assessed through ELISA and qRT-PCR techniques. Interestingly, five previously undescribed metabolites, including a pentadienoic acid derivative, a triterpenoid glycoside, and three tamarixetin glycosides, were identified alongside seven known compounds. Most compounds evaluated had higher DPPH radical scavenging capabilities than the standard, trolox. Tamarixetin 3-O-(alpha-L-rhamnopyranosyl)-(1 -> 2)-beta-D-galactopyranoside (11), a previously undescribed compound, was the most effective at suppressing TNF-alpha protein and m-RNA levels. Other flavonoid glycosides, quercetin 3-O-(alpha-L-rhamnopyranosyl-(1 -> 2)-beta-D-xylopyranoside (4), isovitixin (5), and quercetin-3-O-[alpha-L -rhamnopyranosyl-(1 -> 2)]-[alpha-L-rhamnopyranosyl-(1 -> 6)]-beta-D-galactopyranoside (9), also significantly lowered TNIF-alpha production. [GRAPHICS] .

Automated summary

This study identified several compounds with antioxidant and anti-inflammatory potentials in Adenanthera pavonina, including a previously undescribed compound that showed the strongest inhibition of TNF-alpha production.