期刊
NANO LETTERS
卷 22, 期 1, 页码 50-57出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.nanolett.1c03108
关键词
SARS-CoV-2; variant screening; ACE2; virus receptor; biosensor
类别
资金
- National Research Foundation (NRF) - Ministry of Science and ICT (MSIT) [2020R1A2C2009291]
- Ministry of Education of the Republic of Korea [2020R1A6A3A01100429]
- Korea Medical Device Development Fund - Korean government (the Ministry of Science and ICT) [1711138210, KMDF_PR_20200901_0115-01]
- Korea Medical Device Development Fund - Korean government (Ministry of Trade, Industry and Energy) [1711138210, KMDF_PR_20200901_0115-01]
- Korea Medical Device Development Fund - Korean government (Ministry of Health Welfare) [1711138210, KMDF_PR_20200901_0115-01]
- Korea Medical Device Development Fund - Korean government (Ministry of Food and Drug Safety) [1711138210, KMDF_PR_20200901_0115-01]
- Korea Institute of Science and Technology (KIST) [2E31130, 2E3115C]
- KU-KIST Graduate School of Converging Science and Technology, Korea University
- National Research Foundation of Korea [2020R1A6A3A01100429, 2020R1A2C2009291, 2E31130, KMDF_PR_20200901_0115-01] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Researchers developed a biosensor based on the virus receptor to detect SARS-CoV-2 efficiently and sensitively, which may serve as an effective strategy for screening infectious diseases to prevent pandemics.
SARS-CoV-2 variants are of particular interest because they can potentially increase the transmissibility and virulence of COVID-19 or reduce the effectiveness of available vaccines. However, screening SARS-CoV-2 variants is a challenge because biosensors target viral components that can mutate. One promising strategy is to screen variants via angiotensin-converting enzyme 2 (ACE2), a virus receptor shared by all known SARS-CoV-2 variants. Here we designed a highly sensitive and portable COVID-19 screening biosensor based on the virus receptor. We chose a dual-gate field-effect transistor to overcome the low sensitivity of virus-receptor-based biosensors. To optimize the biosensor, we introduced a synthetic virus that mimics the important features of SARS-CoV-2 (size, bilayer structure, and composition). The developed biosensor successfully detected SARS-CoV-2 in 20 min and showed sensitivity comparable to that of molecular diagnostic tests (similar to 165 copies/mL). Our results indicate that a virus-receptor-based biosensor can be an effective strategy for screening infectious diseases to prevent pandemics.
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