4.5 Article

Evaluation of OIDx Histoplasma Urinary Antigen EIA

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MYCOPATHOLOGIA
卷 187, 期 1, 页码 129-131

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SPRINGER
DOI: 10.1007/s11046-021-00602-9

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Histoplasma; Histoplasmosis; Antigen; Enzyme immunoassay

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The sandwich enzyme immunoassay developed by OIDx for Histoplasma urinary Ag showed high sensitivity, but had a high rate of false-positive results in non-histoplasmosis cases.
A sandwich enzyme immunoassay (EIA) for the detection of Histoplasma antigens (Ag) in urine, developed by Optimum Imaging Diagnostics (OIDx) was evaluated. A verification using a standardized reference panel of urine samples found sensitivity of 92%, specificity of 32% and accuracy of 51%. In this study, the OIDx Histoplasma urinary Ag EIA displayed high sensitivity, however, in non-histoplasmosis cases this EIA displayed false-positive results in 68% of specimens tested.

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