期刊
MOLECULES
卷 27, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/molecules27010122
关键词
eucalyptus leaf extract; fructose absorption; oenothein B; polyphenol; hydrolyzable tannins; ellagitannin; glucose transporter 5; Caco-2 cell; polyvinylpolypyrrolidone
The study found that oenothein B in Eucalyptus leaf extract (ELE) can inhibit fructose absorption without affecting glucose absorption. This discovery has the potential to prevent and improve obesity and other diseases caused by dietary fructose consumption.
Inhibition of fructose absorption may suppress adiposity and adiposity-related diseases caused by fructose ingestion. Eucalyptus leaf extract (ELE) inhibits intestinal fructose absorption (but not glucose absorption); however, its active compound has not yet been identified. Therefore, we evaluated the inhibitory activity of ELE obtained from Eucalyptus globulus using an intestinal fructose permeation assay with the human intestinal epithelial cell line Caco-2. The luminal sides of a cell monolayer model cultured on membrane filters were exposed to fructose with or without the ELE. Cellular fructose permeation was evaluated by measuring the fructose concentration in the medium on the basolateral side. ELE inhibited 65% of fructose absorption at a final concentration of 1 mg/mL. Oenothein B isolated from the ELE strongly inhibited fructose absorption; the inhibition rate was 63% at a final concentration of 5 mu g/mL. Oenothein B did not affect glucose absorption. In contrast, the other major constituents (i.e., gallic acid and ellagic acid) showed little fructose-inhibitory activity. To our knowledge, this is the first report that oenothein B in ELE strongly inhibits fructose absorption in vitro. ELE containing oenothein B can prevent and ameliorate obesity and other diseases caused by dietary fructose consumption.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据