Biosynthesis of Tetrapyrrole Cofactors by Bacterial Community Inhabiting Porphyrine-Containing Shale Rock (Fore-Sudetic Monocline)

This study describes for the first time the comprehensive characterization of tetrapyrrole cofactor biosynthetic pathways developed for bacterial community (BC) inhabiting shale rock. Based on the genomic and proteomic metadata, we have detailed the biosynthesis of siroheme, heme, cobalamin, and the major precursor uroporphyrinogen III by a deep BC living on a rock containing sedimentary tetrapyrrole compounds. The obtained results showed the presence of incomplete heme and cobalamin biosynthesis pathways in the studied BC. At the same time, the production of proteins containing these cofactors, such as cytochromes, catalases and sulfite reductase, was observed. The results obtained are crucial for understanding the ecology of bacteria inhabiting shale rock, as well as their metabolism and potential impact on the biogeochemistry of these rocks. Based on the findings, we hypothesize that the bacteria may use primary or modified sedimentary porphyrins and their degradation products as precursors for synthesizing tetrapyrrole cofactors. Experimental testing of this hypothesis is of course necessary, but its evidence would point to an important and unique phenomenon of the tetrapyrrole ring cycle on Earth involving bacteria.

Automated summary

This study provides a comprehensive characterization of tetrapyrrole cofactor biosynthetic pathways in bacterial communities inhabiting shale rock, revealing incomplete heme and cobalamin biosynthesis pathways and the presence of related proteins. The findings are crucial for understanding the ecology, metabolism, and potential impact of bacteria in shale rock biogeochemistry. The hypothesis of using sedimentary porphyrins as precursors for synthesizing tetrapyrrole cofactors points to a unique phenomenon that involves bacteria in the tetrapyrrole ring cycle on Earth.