期刊
MOLECULES
卷 26, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/molecules26196052
关键词
HPLC; method validation; Miang; catechins; caffeine; gallic acid
资金
- Agricultural Research Development Agency (ARDA), Thailand
- National Research Council of Thailand (NRCT) [PRP5805021460]
- Cluster of Excellence on Biodiversity-based Economic and Society (B.BES-CMU), Chiang Mai University, Thailand
The study developed an HPLC method for detecting catechins and related compounds in Mieng, overcoming matrix issues. Seven catechins were identified, and a reproducible analysis system was developed.
High performance liquid chromatography (HPLC) for catechins and related compounds in Miang (traditional Lanna fermented tea leaf) was developed to overcome the matrices during the fermentation process. We investigated a variety of columns and elution conditions to determine seven catechins, namely (+)-catechin, (-)-gallocatechin, (-)-epigallocatechin, (-)-epicatechin, (-)-epigallocatechin gallate, (-)-gallocatechin gallate, (-)-epicatechin gallate, as well as gallic acid and caffeine, resulting in the development of reproducible systems for analyses that overcome sample matrices. Among the three reversed-phase columns, column C (deactivated, with extra dense bonding, double endcapped monomeric C18, high-purity silica at 3.0 mm x 250 mm and a 5 mu m particle size) significantly improved the separation between Miang catechins in the presence of acid in the mobile phase within a shorter analysis time. The validation method showed effective linearity, precision, accuracy, and limits of detection and quantitation. The validated system was adequate for the qualitative and quantitative measurement of seven active catechins, including gallic acid and caffeine in Miang, during the fermentation process and standardization of Miang extracts. The latter contain catechins and related compounds that are further developed into natural active pharmaceutical ingredients (natural APIs) for cosmeceutical and nutraceutical products.
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