期刊
MOLECULES
卷 26, 期 19, 页码 -出版社
MDPI
DOI: 10.3390/molecules26195788
关键词
NMR spectroscopy; gold nanoparticles; PEGylation; adsorption; passivation
资金
- National Institute of Allergy and Infectious Diseases of the National Institutes of Health [R01AI139479]
- National Science Foundation [1818090, 1852527]
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1818090] Funding Source: National Science Foundation
- Direct For Mathematical & Physical Scien
- Division Of Chemistry [1852527] Funding Source: National Science Foundation
The study focused on examining the adsorption amount of biomolecules on PEGylated gold nanoparticles, revealing that cysteine residues can dramatically increase adsorption rates, and shorter chain PEG molecules are more effective in protecting against all protein types.
Polyethylene glycol (PEG) surface conjugations are widely employed to render passivating properties to nanoparticles in biological applications. The benefits of surface passivation by PEG are reduced protein adsorption, diminished non-specific interactions, and improvement in pharmacokinetics. However, the limitations of PEG passivation remain an active area of research, and recent examples from the literature demonstrate how PEG passivation can fail. Here, we study the adsorption amount of biomolecules to PEGylated gold nanoparticles (AuNPs), focusing on how different protein properties influence binding. The AuNPs are PEGylated with three different sizes of conjugated PEG chains, and we examine interactions with proteins of different sizes, charges, and surface cysteine content. The experiments are carried out in vitro at physiologically relevant timescales to obtain the adsorption amounts and rates of each biomolecule on AuNP-PEGs of varying compositions. Our findings are relevant in understanding how protein size and the surface cysteine content affect binding, and our work reveals that cysteine residues can dramatically increase adsorption rates on PEGylated AuNPs. Moreover, shorter chain PEG molecules passivate the AuNP surface more effectively against all protein types.
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