期刊
MOLECULES
卷 26, 期 22, 页码 -出版社
MDPI
DOI: 10.3390/molecules26226797
关键词
ion-pair HPLC-UV method; validation; in vivo application; iodine supplementation; pregnancy
资金
- Polish National Centre for Science [PRELUDIUM-17]
- Poznan University of Medical Sciences [2019/33/N/NZ5/02303]
A novel ion-pair HPLC-UV method was developed for measuring iodine concentration in clinical samples, showing sufficient precision and accuracy. The study revealed that urinary iodine concentration in pregnant women was influenced by iodine supplementation, with the non-supplemented group having lower levels compared to the supplemented group.
Iodine is an essential component for fetal neurodevelopment and maternal thyroid function. Urine iodine is the most widely used indicator of iodine status. In this study, a novel validated ion-pair HPLC-UV method was developed to measure iodine concentration in clinical samples. A sodium thiosulfate solution was added to the urine sample to convert the total free iodine to iodide. Chromatographic separation was achieved in a Pursuit XRs C8 column. The mobile phase consisted of acetonitrile and a water phase containing 18-crown-6-ether, octylamine and sodium dihydrogen phosphate. Validation parameters, such as accuracy, precision, limits of detection and quantification, linearity and stability, were determined. Urinary samples from pregnant women were used to complete the validation and confirm the method's applicability. In the studied population of 93 pregnant women, the median UIC was lower in the group without iodine supplementation (117 mu g/L, confidence interval (%CI): 95; 138) than in the supplement group (133 mu g/L, %CI: 109; 157). In conclusion, the newly established ion-pair HPLC-UV method was adequately precise, accurate and fulfilled validation the criteria for analyzing compounds in biological fluids. The method is less complicated and expensive than other frequently used assays and permits the identification of the iodine-deficient subjects.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据