4.6 Article

Phosphate Starvation by Energy Metabolism Disturbance in Candida albicans vip1Δ/Δ Induces Lipid Droplet Accumulation and Cell Membrane Damage

期刊

MOLECULES
卷 27, 期 3, 页码 -

出版社

MDPI
DOI: 10.3390/molecules27030686

关键词

PHO pathway; phosphate stress; lipid droplet

资金

  1. National Natural Science Foundation of China [32070145, 81873961, 31870139, 81973581]
  2. Natural Science Foundation of Tianjin [19JCZDJC33800]

向作者/读者索取更多资源

This study investigates the role of the vip1 gene in response to phosphate stress and lipid metabolism in yeast. The results show that the deletion of the vip1 gene leads to lipid droplet accumulation and cell membrane damage. However, supplementing phosphate or overexpressing phosphate transporters does not affect the energy balance in vip1 mutant strains. Furthermore, the study finds that the vip1 mutant strains have reduced activity in the PHO pathway.
Phosphorus in the form of phosphate (Pi) is an essential element for metabolic processes, including lipid metabolism. In yeast, the inositol polyphosphate kinase vip1 mediated synthesis of inositol heptakisphosphate (IP7) regulates the phosphate-responsive (PHO) signaling pathway, which plays an important role in response to Pi stress. The role of vip1 in Pi stress and lipid metabolism of Candida albicans has not yet been studied. We found that when vip1 Delta/Delta was grown in glucose medium, if Pi was supplemented in the medium or mitochondrial Pi transporter was overexpressed in the strain, the lipid droplet (LD) content was reduced and membrane damage was alleviated. However, further studies showed that neither the addition of Pi nor the overexpression of the Pi transporter affected the energy balance of vip1 Delta/Delta. In addition, the LD content of vip1 Delta/Delta grown in Pi limitation medium PNMC was lower than that grown in SC, and the metabolic activity of vip1 Delta/Delta grown in PNMC was also lower than that grown in SC medium. This suggests that the increase in Pi demand by a high energy metabolic rate is the cause of LD accumulation in vip1 Delta/Delta. In addition, in the vip1 Delta/Delta strains, the core transcription factor PHO4 in the PHO pathway was transported to the vacuole and degraded, which reduced the pathway activity. However, this does not mean that knocking out vip1 completely blocks the activation of the PHO pathway, because the LD content of vip1 Delta/Delta grown in the medium with beta-glycerol phosphate as the Pi source was significantly reduced. In summary, the increased Pi demand and the decreased PHO pathway activity in vip1 Delta/Delta ultimately lead to LD accumulation and cell membrane damage.

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