期刊
MOLECULES
卷 27, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/molecules27041329
关键词
carrageenan; MIC; NO; PGE-2; TNF-alpha; Yucca elephantipes; LC-MS/MS
资金
- Princess Nourah bint Abdulrahman University Researchers Supporting Project [PNURSP2022R204]
- Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
This study investigated the anti-inflammatory activity of Yucca gigantea methanol extract (YGME) and its possible mechanism against carrageenan-induced acute inflammation. The phytochemical composition, cytotoxicity, and antimicrobial activity of YGME were also explored. The results showed that YGME exhibited significant anti-inflammatory effects and good cytotoxicity against tumor cells, as well as potent antimicrobial activity.
The acute inflammation process is explained by numerous hypotheses, including oxidative stress, enzyme stimulation, and the generation of pro-inflammatory cytokines. The anti-inflammatory activity of Yucca gigantea methanol extract (YGME) against carrageenan-induced acute inflammation and possible underlying mechanisms was investigated. The phytochemical profile, cytotoxic, and antimicrobial activities were also explored. LC-MS/MS was utilized to investigate the chemical composition of YGME, and 29 compounds were tentatively identified. In addition, the isolation of luteolin-7-O-beta-d-glucoside, apigenin-7-O-beta-d-glucoside, and kaempferol-3-O-alpha-l-rhamnoside was performed for the first time from the studied plant. Inflammation was induced by subcutaneous injection of 100 mu L of 1% carrageenan sodium. Rats were treated orally with YGME 100, 200 mg/kg, celecoxib (50 mg/kg), and saline, respectively, one hour before carrageenan injection. The average volume of paws edema and weight were measured at several time intervals. Levels of NO, GSH, TNF-alpha, PGE-2, serum IL-1 beta, IL-6 were measured. In additionally, COX-2 immunostaining and histopathological examination of paw tissue were performed. YGME displayed a potent anti-inflammatory influence by reducing paws edema, PGE-2, TNF-alpha, NO production, serum IL-6, IL-1 beta, and COX-2 immunostaining. Furthermore, it replenished the diminished paw GSH contents and improved the histopathological findings. The best cytotoxic effect of YGME was against human melanoma cell line (A365) and osteosarcoma cell line (MG-63). Moreover, the antimicrobial potential of the extract was evaluated against bacterial and fungal isolates. It showed potent activity against Gram-negative, Gram-positive, and fungal Candida albicans isolates. The promoting multiple effects of YGME could be beneficial in the treatment of different ailments based on its anti-inflammatory, antimicrobial, and cytotoxic effects.
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