期刊
MOLECULES
卷 27, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/molecules27041254
关键词
Panax notoginseng polysaccharides; pseudorabies virus (PRV); antiviral; infection; adsorption; replication
资金
- National Natural Science Foundation of China [31902253]
- Natural Science Foundation of Jiangsu Province [BK20180921]
- individual technology research and development of modern agricultural industry of Jiangsu Province [CX(19)3024]
- China Postdoctoral Science Foundation [2018M632399]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Top-level Talents Support Program of Yangzhou University
Panax notoginseng polysaccharides exhibit antiviral activity against pseudorabies virus by inhibiting virus adsorption and replication.
Porcine pseudorabies (PR) is an important infectious disease caused by pseudorabies virus (PRV), which poses a major threat to food safety and security. Vaccine immunization has become the main means to prevent and control the disease. However, since 2011, a new PRV variant has caused huge economic losses to the Chinese pig industry. Panax notoginseng polysaccharides have immunomodulatory activity and other functions, but the antiviral effect has not been reported. We studied the anti-PRV activity of Panax notoginseng polysaccharides in vitro. A less cytopathic effect was observed by increasing the concentration of Panax notoginseng polysaccharides. Western blot, TCID50, plaque assay, and IFA revealed that Panax notoginseng polysaccharides could significantly inhibit the infectivity of PRV XJ5 on PK15 cells. In addition, we also found that Panax notoginseng polysaccharides blocked the adsorption and replication of PRV to PK15 cells in a dose-dependent manner. These results show that Panax notoginseng polysaccharides play an antiviral effect mainly by inhibiting virus adsorption and replication in vitro. Therefore, Panax notoginseng polysaccharides may be a potential anti-PRV agent.
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