期刊
MOLECULAR THERAPY
卷 30, 期 3, 页码 1227-1238出版社
CELL PRESS
DOI: 10.1016/j.ymthe.2021.12.013
关键词
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资金
- Chongqing Special Project for Academicians [cstc2020yszx-jcyjX0004]
- Projects of the State Key Laboratory of Trauma, Burns and Combined Injury [SKLYQ201901]
- Training Plan for Innovation Ability on the Frontiers of Military Medical Research [2019CXJSB014]
- Chongqing Special Project for Epidemic Prevention Doctors [2020FYYX239]
Immunosuppression in response to severe sepsis is a significant concern in human health. CD4 T cells, particularly those expressing Tim-3, play a critical role in sepsis-induced immunosuppression. Tim-3 inhibits the NF-kappa B signaling pathway in CD4 T cells during sepsis, contributing to the immunosuppressive effect.
Immunosuppression in response to severe sepsis remains a serious human health concern. Evidence of sepsis-induced immunosuppression includes impaired T lymphocyte function, T lymphocyte depletion or exhaustion, increased susceptibility to opportunistic nosocomial infection, and imbalanced cytokine secretion. CD4 T cells play a critical role in cellular and humoral immune responses during sepsis. Here, using an RNA sequencing assay, we found that the expression of T cell-containing immunoglobulin and mucin domain-3 (Tim-3) on CD4 T cells in sepsis-induced immunosuppression patients was significantly elevated. Furthermore, the percentage of Tim-3(+) CD4 T cells from sepsis patients was correlated with the mortality of sepsis-induced immunosuppression. Conditional deletion of Tim-3 in CD4 T cells and systemic Tim-3 deletion both reduced mortality in response to sepsis in mice by preserving organ function. Tim-3(+) CD4 T cells exhibited reduced proliferative ability and elevated expression of inhibitory markers compared with Tim-3(-) CD4 T cells. Colocalization analyses indicated that HMGB1 was a ligand that binds to Tim-3 on CD4 T cells and that its binding inhibited the NF-kappa B signaling pathway in Tim-3(+) CD4 T cells during sepsis-induced immunosuppression. Together, our findings reveal the mechanism of Tim-3 in regulating sepsis-induced immunosuppression and provide a novel therapeutic target for this condition.
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