期刊
MOLECULAR PLANT PATHOLOGY
卷 23, 期 1, 页码 133-147出版社
WILEY
DOI: 10.1111/mpp.13149
关键词
actin ring; appressorium formation; autophagy; cAMP pathway; endocytosis; Pmk1 pathway
资金
- National Natural Science Foundation of China [31871909, 32072365]
- Open Research Fund of the State Key Laboratory of Hybrid Rice (Hunan Hybrid Rice Research Center) [2019KF04]
The PAL1 gene plays a crucial role in endocytosis in the plant-pathogenic fungus Magnaporthe oryzae, regulating appressorium formation and maturation. Through interactions with endocytosis proteins and modulation of cAMP and Pmk1 signaling pathways, Pal1 influences various cellular processes critical for successful infection.
Endocytosis plays key roles during infection of plant-pathogenic fungi, but its regulatory mechanisms are still largely unknown. Here, we identified a putative endocytosis-related gene, PAL1, which was highly expressed in appressorium of Magnaporthe oryzae, and was found to be important for appressorium formation and maturation. Deletion of PAL1 significantly reduced the virulence of M. oryzae due to defects in appressorial penetration and invasive growth in host cells. The Pal1 protein interacted and colocalized with the endocytosis protein Sla1, suggesting it is involved in endocytosis. The Delta pal1 mutant was significantly reduced in appressorium formation, which was recovered by adding exogenous cAMP and 3-isobutyl-1-methylxanthine (IBMX). Moreover, the phosphorylation level of Pmk1 in Delta pal1 was also reduced, suggesting Pal1 functions upstream of both the cAMP and Pmk1 signalling pathways. As a consequence, the utilization of glycogen and lipid, appressorial autophagy, actin ring formation, localization of septin proteins, as well as turgor accumulation were all affected in the Delta pal1 mutant. Taken together, Pal1 regulates cAMP and the Pmk1 signalling pathway for appressorium formation and maturation to facilitate infection of M. oryzae.
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