期刊
MOLECULAR ONCOLOGY
卷 16, 期 6, 页码 1347-1364出版社
WILEY
DOI: 10.1002/1878-0261.13125
关键词
3 ' UTR; APA; cancer cell senescence; CDK16; miR-485-5p
类别
资金
- National Key R&D Program of China [2018YFC1003500]
- National Natural Science Foundation of China [91949107, 31771336, 31521003]
- Shanghai Municipal Science and Technology Major Project [2017SHZDZX01]
The study discovered that the expression of CDK16 is regulated by APA and miR-485-5p, making it a potential target for prosenescence therapy for lung cancer.
Inducing senescence in cancer cells is an emerging strategy for cancer therapy. The dysregulation and mutation of genes encoding cyclin-dependent kinases (CDKs) have been implicated in various human cancers. However, whether CDK can induce cancer cell senescence remains poorly understood. We observed that CDK16 expression was high in multiple cancer types, including lung cancer, whereas various replicative senescence models displayed low CDK16 expression. CDK16 knockdown caused senescence-associated phenotypes in lung cancer cell lines. Interestingly, the CDK16 3' UTR was shortened in cancer and lengthened in senescence models, which was regulated by alternative polyadenylation (APA). The longer 3'UTR [using the distal polyA (pA) site] generated less protein than the shorter one (using the proximal pA site). Since microRNAs (miRNAs) usually bind to the 3'UTR of target genes to suppress their expression, we investigated whether miRNAs targeting the region between the shortened and longer 3'UTR are responsible for the reduced expression. We found that miR-485-5p targeted the 3'UTR between the distal and proximal pA site and caused senescence-associated phenotypes by reducing protein production from the longer CDK16 transcript. Of note, CDK16 knockdown led to a reduced expression of MYC proto-oncogene, bHLH transcription factor (MYC) and CD274 molecule (PD-L1), which in turn enhanced the tumor-suppressive effects of senescent cancer cells. The present study discovered that CDK16, whose expression is under the regulation of APA and miR-485-5p, is a potential target for prosenescence therapy for lung cancer.
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