期刊
MOLECULAR NUTRITION & FOOD RESEARCH
卷 66, 期 7, 页码 -出版社
WILEY
DOI: 10.1002/mnfr.202101115
关键词
ceramides; diacylglycerols; fatty acid beta-oxidation; lipidomics; liver steatosis; NAFLD
资金
- MCIN/AEI [SAF2017-82369-R]
- ERDF A way of making Europe, Generalitat de Catalunya [2017 SGR 38]
- Government of Paraguay [BCAL04-327]
- Instituto de Salud Carlos III Miguel Servet fellowship [CP II 17/00029]
This study aims to determine the role of dietary saturated fatty acids (FA) versus liquid fructose in the development of fatty liver and hypertriglyceridemia. The results show that adding liquid fructose to the diet plays a decisive role in the accumulation of liver fat and production of high triglycerides, through increased de novo lipogenesis and reduced fatty acid catabolism.
Scope: The aim of this study is to delineate the contribution of dietary saturated fatty acids (FA) versus liquid fructose to fatty liver and hypertriglyceridemia. Methods and Results: Three groups of female rats are maintained for 3 months in standard chow (CT); High-fat diet (46.9% of fat-derived calories, rich in palmitic and stearic FA, HFD); and HFD with 10% w/v fructose in drinking water (HFHFr). Zoometric parameters, plasma biochemistry, and liver Oil-Red O (ORO) staining, lipidomics, and expression of proteins involved in FA metabolism are analyzed. Both diets increase ingested calories without modifying body weight. Only the HFHFr diet increases liver triglycerides (x11.0), with hypertriglyceridemia (x1.7) and reduces FA beta-oxidation (x0.7), and increases liver FA markers of DNL (de novo lipogenesis). Whereas HFD livers show a high content of ceramides, HFHFr samples show unchanged ceramides, and an increase in diacylglycerols. Only the HFHFr diet leads to a marked increase in the expression of enzymes involved in DNL and triglyceride metabolism, such as carbohydrate response element binding protein beta (ChREBP beta, x3.2), a transcription factor that regulates DNL, and patatin-like phospholipase domain-containing 3 (PNPLA3, x2.6), a lipase that mobilizes stored triglycerides for VLDL secretion. Conclusion: The addition of liquid-fructose to dietary FA is determinant in liver steatosis and hypertriglyceridemia production, through increased DNL and PNPLA3 expression, and reduced FA catabolism.
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