Biochanin A Sensitizes Glioblastoma to Temozolomide by Inhibiting Autophagy

Resistance to temozolomide (TMZ) chemotherapy is the main reason for treatment failure in patients with glioblastoma (GBM). In the present study, we investigated biochanin A (BCA) a potent sensitizer of TMZ in GBM. We observed that BCA significantly enhanced cell sensitivity to TMZ in vitro and in vivo. Mechanistically, the specific chemosensitizing effect of BCA is mediated by autophagy inhibition. Moreover, by performing a molecular docking analysis, we demonstrated that BCA interacts with AMPK residues and impairs autophagy by regulating the AMPK/ULK1 pathway. These results suggest that BCA is a potential therapeutic agent that sensitizes GBM to TMZ and provide new insight into its therapeutic potential in chemoresistant GBM.

Automated summary

The study found that BCA can effectively enhance sensitivity of GBM to TMZ by inhibiting autophagy, mediated through interaction with AMPK residues. This discovery sheds new light on the potential therapeutic role of BCA in sensitizing chemoresistant GBM to TMZ.