期刊
MOLECULAR MICROBIOLOGY
卷 117, 期 3, 页码 578-588出版社
WILEY
DOI: 10.1111/mmi.14817
关键词
ex vivo; infection; organotypic models; precision-cut lung slices; respiratory infections
资金
- H2020 Marie Sklodowska-Curie Actions [844197]
- Marie Curie Actions (MSCA) [844197] Funding Source: Marie Curie Actions (MSCA)
Research on the mechanisms of respiratory infections is often limited by the current models used. Precision-cut lung slices (PCLS) offer a promising ex vivo organotypic model that retains the cellular complexity and architecture of the lung, providing a platform for investigating respiratory pathogens in a near-native environment.
Respiratory infections are a leading cause of mortality worldwide. Most of the research on the underlying disease mechanisms is based on cell culture, organoid, or surrogate animal models. Although these provide important insights, they have limitations. Cell culture models fail to recapitulate cellular interactions in the lung and animal models often do not permit high-throughput analysis of drugs or pathogen isolates; hence, there is a need for improved, scalable models. Precision-cut lung slices (PCLS), small, uniform tissue slices generated from animal or human lungs are increasingly recognized and employed as an ex vivo organotypic model. PCLS retain remarkable cellular complexity and the architecture of the lung, providing a platform to investigate respiratory pathogens in a near-native environment. Here, we review the generation and features of PCLS, their use to investigate the pathogenesis of viral and bacterial pathogens, and highlight their potential to advance respiratory infection research in the future.
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