期刊
MOLECULAR CELL
卷 82, 期 2, 页码 447-+出版社
CELL PRESS
DOI: 10.1016/j.molcel.2021.11.006
关键词
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资金
- American Diabetes Association [1-18-PDF-144]
- Austrian Science Fund [FWF W1226, FWF P27108]
- NIH [T32 GM07170, T32 GM-07229, 2-T32-CA115299-13, F99CA222741, 1 S10 OD030286-01]
- Leukemia Research Foundation (Hollis Brownstein New Investigator Research Grant)
- AFAR (Sagol Network GerOmic Award)
- Deerfield (Xseed award)
- NIHP30 grant [CA01333047]
- [R01GM132261]
- [P30ES013508]
- [R01CA228339]
- [R01DK116005]
- [R01CA174761]
- [R01CA248315]
This study developed a stable isotope labeling technique for quantifying acyl-CoA thioesters in subcellular compartments. The researchers found that oxygen tension, cellular differentiation, and nutrient availability have compartmentalized effects on subcellular metabolism. Importantly, they discovered a significant enrichment of propionyl-CoA in the nuclear compartment and identified isoleucine as a major metabolic source for nuclear propionyl-CoA and histone propionylation, revealing a new mechanism of crosstalk between metabolism and the epigenome.
Quantitative subcellular metabolomic measurements can explain the roles of metabolites in cellular processes but are subject to multiple confounding factors. We developed stable isotope labeling of essential nutrients in cell culture-subcellular fractionation (SILEC-SF), which uses isotope-labeled internal standard controls that are present throughout fractionation and processing to quantify acyl-coenzyme A (acyl-CoA) thioesters in subcellular compartments by liquid chromatography-mass spectrometry. We tested SILEC-SF in a range of sample types and examined the compartmentalized responses to oxygen tension, cellular differentiation, and nutrient availability. Application of SILEC-SF to the challenging analysis of the nuclear compartment revealed a nuclear acyl-CoA profile distinct from that of the cytosol, with notable nuclear enrichment of propionyl-CoA. Using isotope tracing, we identified the branched chain amino acid isoleucine as a major metabolic source of nuclear propionyl-CoA and histone propionylation, thus revealing a new mechanism of crosstalk between metabolism and the epigenome.
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