4.5 Article

Development and characterization of novel microsatellite markers in Tilia amurensis Rupr. using next-generation sequencing

期刊

MOLECULAR BIOLOGY REPORTS
卷 49, 期 2, 页码 1637-1641

出版社

SPRINGER
DOI: 10.1007/s11033-021-07035-z

关键词

Tilia amurensis; Next-generation sequencing; Microsatellite marker; Genetic diversity

资金

  1. National Institute of Forest Science of the Republic of Korea [FG0601-2019-01]

向作者/读者索取更多资源

This study developed and characterized novel microsatellite markers of Tilia amurensis, and characterized three populations in Korea, demonstrating the significance of these markers for genetic diversity studies.
Background Tilia amurensis (Malvaceae) is a deciduous broad-leaved tree distributed in Korea, China, and Japan. T. amurensis is used as a honey tree and also as a material for furniture, carving, and pulp. This study aimed to develop and characterize novel microsatellite markers using next-generation sequencing (NGS) of T. amurensis. Methods and results NGS analysis using GS-FLX Titanium obtained 629,273 reads, of which 15,795 contigs were assembled with an average length of 830 bp. A total of 4774 microsatellite regions were detected in 3602 of 15,795 contigs. In total, 360 primer sets were designed based on the microsatellite regions. Among them, 15 primer sets were selected as reproducible polymorphic markers and were characterized for three populations of T. amurensis in Korea. The average number of alleles (N-A) was 3.5 in Mt. Hambaek (HB), 3.7 in Mt. Odae (OD), and 3.8 in Mt. Sobaek (SB). The average observed heterozygosity (H-O) and expected heterozygosity (H-E) values were 0.497 and 0.370 in the HB population, 0.470 and 0.372 in the OD population, and 0.524 and 0.410 in the SB population, respectively. The average polymorphic information content (PIC) value of the 15 microsatellite markers was 0.686. Conclusions The novel microsatellite markers will be useful for further studies on genetic diversity evaluation to conserve the genetic resources and natural populations of T. amurensis.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.5
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据