期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 83, 期 -, 页码 235-240出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2015.11.077
关键词
Rose pepper; AAI; MIC; Aroeira; Urinary tract infection
资金
- Fundacao de Amparo a Pesquisa do Espirito Santo (FAPES)
The leaves of Schinus terebinthifolius Raddi (Anacardiaceae), the Brazilian pepper tree, are widely used in folk medicine for treating human diseases caused by microorganisms. The aim of the present study was to investigate the antioxidant and antimicrobial activity and the chemical composition of S. terebinthifolius leaf extract and essential oil. Essential oil from fresh leaves of S. terebinthifolius was obtained in a Clevenger-type apparatus. Ethanol leaves extracts were prepared by maceration and ultrasound assisted extraction. Antimicrobial activity was tested at concentrations up to 1000 mu g/ml by the microdilution test after 24 h and antioxidant activity was investigated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging assay. Phenolic compounds were determined by the Folin Cioucalteu method and flavonoids by the aluminum chloride technique. The chemical composition of the essential oil and the extracts were characterized by GC/MS and LC MS/MS, respectively. Ultrasound assisted extraction exhibited higher yield (182.25 +/- 3.00 mg/g) and flavonoid content (243.09 +/- 8.83 mg/g), while the extraction method of maceration showed higher content of total phenolic compounds (221.63 +/- 2.58 mg/g) and exhibited strong antioxidant activity (IC50 15.43 mu g/ml). All extracts showed strong antimicrobial activity against Staphylococcus aureus and Escherichia coll. Thirty-two constituents were identified in the oil (97.90% of the total), with delta-3-carene (68.78%), E-caryophyllene (8.22%), myrcene (6.78%) and alpha-pinene (4.05%) as the main compounds. Mass spectrometry analyzes revealed that ferulic and caffeic acids, and quercetin were the major components in the extracts. In summary, these findings demonstrate the potential antioxidant and antimicrobial activity of extracts that may help to explain the popular use for urinary tract infection of S. terebinthifolius. (c) 2015 Elsevier B.V. All rights reserved.
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