期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 83, 期 -, 页码 372-378出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2016.01.013
关键词
Jerusalem artichoke; Docosahexaenoic acid (DHA); Aurantiochytrium sp.; Osmotic pressure; Micronutrient; Response surface methodology
资金
- Zhejiang Provincial Natural Science Foundation of China [LQ13C010002, LQ14C010002]
- Natural Science Foundation of Zhejiang University of Technology [2013XZ007]
- Research Foundation of Zhejiang Education Department [Y201225077]
Jerusalem artichoke was first investigated as a non-food and cheap feedback for the production of docosahexaenoic acid (DHA) through Aurantiochytrium sp. YLH70. Using the Response surface method, up to 143.8 g/L. of reducing sugar was obtained in the acidic hydrolysate of Jerusalem artichoke at 80 degrees C and 6% (v/v) sulphuric acid after 40 min of hydrolysis. The Plackett-Burman experiment showed that natural components of the hydrolysate of Jerusalem artichoke without the addition of any micronutrient, which is essential in traditional media, were suitable for DHA production by Aurantiochytrium sp. YLH70. Moreover, compared to the fructose or glucose medium, the Jerusalem artichoke medium resulted in a higher biomass (32.71 g/L) and DHA content (46.9% of the total fatty acid), which could be associated with the rich nutrition and high osmotic pressure (1306 mOsm/kg H2O) in the Jerusalem artichoke medium. During fed-batch fermentation under the optimal Jerusalem artichoke medium (695 mL/L hydrolysate of Jerusalem artichoke, 15 g/L yeast extract and 25 g/L sea salt), both a productivity of 3.35 g/L/d and a yield of 0.094 g/g reducing sugar for DHA production were achieved. These results demonstrate that Jerusalem artichoke, when sufficiently utilised by Aurantiochytrium sp. YLH70, could indeed be an efficient and cheap option for DHA production. (C) 2016 Elsevier B.V. All rights reserved.
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