期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 90, 期 -, 页码 9-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.indcrop.2016.06.007
关键词
Ulex europaeus; Isoflavones; Liquiritigenin; HPLC-ESI/MSn analysis; Antioxidant activity; Digestive enzymes inhibition
资金
- Fundacao para a Ciencia e a Tecnologia (FCT, Portugal) [SFRH/BD/84672/2012]
- UCLM Research Plan
- FCT
- Portuguese Government [PEst-OE/QUI/UI0674/2013]
- Portuguese National Mass Spectrometry Network [RNEMREDE/1508/REM/2005]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/84672/2012] Funding Source: FCT
The screening and quantification of the main phenolic compounds in leaves and flowers of Ulex europaeus (gorse) was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometric detection (HPLC-ESI-MSn) after ultrasound-assisted extraction with methanol. About 98% of compounds corresponded to flavonoids, distributed as flavonols, flavones, isoflavones and flavanones. Flavonols were mainly quercetin glucosides; most of the found flavones were apigenin derivatives and the isoflavone group was dominated by glycitin. The flavanone group was composed mainly of liquiritigenin derivatives, substances usually found in liquorice (Glycyrrhiza ssp) and associated with high pharmacological relevance; in Ulex they represent about 25% of total polyphenols content. Phenolic acids and saponins were also detected, as minor components. In vitro antioxidant activity (nitric oxide, superoxide assays, ABTS and DPPH assays) of leaves and flowers, and their inhibitory effects towards digestive enzymes related to carbohydrate metabolism (alpha-glucosidase and alpha-amylase) were also studied. (C) 2016 Elsevier B.V. All rights reserved.
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