Glucose oxidase@zinc-doped zeolitic imidazolate framework-67 as an effective cascade catalyst for one-step chemiluminescence sensing of glucose

A chemiluminescence (CL) sensor was constructed for the one-step determination of glucose. Glucose oxidase (GOx) was successfully encapsulated into Zn-doped zeolitic imidazolate framework-67 (Zn-ZIF-67) via a simple one-pot strategy. The as-prepared GOx@Zn-ZIF-67 nanocomposite can trigger cascade reactions of glucose oxidation to generate H2O2 and H2O2 -mediated luminol reaction to give an intense CL emission. The sensor responds linearly to glucose in the 20.0400.0 mu mol.L-1 range with a limit of detection (LOD) of 4.7 mu mol.L-1. Eleven replicated measurements of 200.0 mu mol.L-1 glucose solution gives a relative standard deviation (RSD) of 1.7%. The sensor exhibits good selectivity and stability and was successfully applied to the determination of glucose in real human serum samples.

Automated summary

A chemiluminescence sensor was developed for one-step determination of glucose by encapsulating glucose oxidase into Zn-doped zeolitic imidazolate framework-67. The sensor showed linear response and low limit of detection for glucose measurement, with good selectivity and stability in real human serum samples.