Ionic liquid-based dispersive liquid-liquid microextraction followed by magnetic solid-phase extraction for determination of quinolones

An ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) combined with magnetic solid-phase extraction (MSPE) was developed for extraction of quinolones (quinolones) from honey and milk prior to high-performance liquid chromatography (HPLC) analysis. 1-Butyl-3-methylimidazolium hexafluorophosphate was used as the extraction solvent and an effective adsorbent based on chitosan modified magnetic core-shell functionalized multi-walled carbon nanotube (MWCNTs-Fe3O4@SiO2-CS) nanoparticles was used to assist IL to adsorb quinolone residues in honey and milk samples. Extraction conditions were optimized through one-factor-at-a-time and response surface methodology using a Box-Behnken design. Under optimum conditions satisfactory linearity (R-2 > 0.999) and high sensitivity (method limits of quantification were 4-8 mu g kg(-1) or mu g L-1 in honey or milk samples) was achieved. The recoveries of quinolones in honey and milk ranged from 81.2 to 109%. Based on this study, the proposed method was employed for the determination of antibiotic residues in honey and milk samples.

Automated summary

An ionic liquid-based dispersive liquid-liquid microextraction combined with magnetic solid-phase extraction method was developed for extraction of quinolones from honey and milk. Satisfactory linearity and high sensitivity were achieved under optimized conditions. The proposed method can be employed for the determination of antibiotic residues in honey and milk samples.