期刊
MICROCHEMICAL JOURNAL
卷 177, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2022.107287
关键词
Polyethyleneimine decorated silver-graphene; oxide nanocomposites; Gold nanoparticles; Electrochemical immunosensor; Olaquindox
资金
- National Key Research and Development Plan of China [2018YFC1602902]
- National Key Research and Development Program of China [2019YFC1604501]
A label-free electrochemical immunosensor was developed for the detection of OLA in feedstuffs using graphene oxide nanocomposites decorated by silver nanoparticles. The sensor showed good stability and sensitivity, with a linear correlation between signal intensity and OLA concentration. The sensor has potential applications for detecting other additives in feedstuffs.
For the first time, a label-free electrochemical immunosensor was developed to detect olaquindox (OLA) in feedstuffs using graphene oxide (GO) nanocomposites (GO-PEI-Ag) decorated by silver nanoparticles (AgNPs) and functionalized by polyethyleneimine (PEI) as electrode modification materials. In this work, GO with huge specific surface area was used as a carrier to load abundant AgNPs, which exhibited good redox activity and perfect conductivity. The stability of GO-Ag in solution was ameliorated by PEI, which was utilized as a solubilizer. Nafion (Nf) was applied to attach tightly and uniformly GO-PEI-Ag nanomaterials on the electrode surface. The gold nanoparticles (AuNPs) were electrodeposited on the GO-PEI-Ag-Nf modified electrode surface for signal amplification and antibody immobilization. Above all, the electrochemical signal significantly improved. Under the optimal conditions, the signal intensity showed linear correlation with the logarithm of OLA concentration ranging from 0.01 ng mL-1 to 150 ng mL(-1), along with the low detection limit of 4.8 pg mL(-1). Moreover, the analytical performance in feedstuff samples was evaluated, showing favorable recovery of 90.0%100.2% with RSD of 2.2%-6.9%. The fabricated biosensor can provide a suitable approach to measure OLA and is promising to detect other additives in feedstuffs.
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