期刊
INDUSTRIAL CROPS AND PRODUCTS
卷 80, 期 -, 页码 148-155出版社
ELSEVIER
DOI: 10.1016/j.indcrop.2015.11.039
关键词
Ionic liquids; Nothofagus pumilio; Eucalyptus globulus Labill; Enzymatic saccharification; Simultaneous Saccharification and; Fermentation; Second generation bioethanol
资金
- CONICYT
- Optimal production of bioethanol from macroalgae via photo-chemo-enzymatic processing (OPTIFU) [AKA-ERNC 0009]
- Centre for Biotechnology and Bioengineering (CeBiB) [FB-0001]
- Departamento de Investigacion VID of University of Chile
- Abo Akademi University
- Academy of Finland [125,113, 138,448]
- Alice and Knut Wallenberg Foundation
- Bio4Energy program
The depletion of petroleum reserves and the high level of pollution caused by fossil fuels have led to enhancing renewable energy and fuel production from biomass. Eucalyptus globulus and Nothofagus pumilio residues could constitute an interesting source of biomass for second generation biofuel production. Lenga residues were pretreated with the ionic liquid (IL) 1-N-ethy1-3-methylimidazolium chloride (C(2)mimCl), followed by subsequent fermentation using both the strategy of Simultaneous Saccharification and Fermentation (SSF) as well as Separate Hydrolysis and Fermentation (SHF). The SHF process yielded 0.134 g ethanol/g glucose (263 wt-% of the theoretical yield) compared to the SSF process which yielded 0.173 g ethanol/g glucose (33.9 wt-% of the theoretical yield) within the first 24h of fermentation. In case of Eucalyptus residues, another IL, 1-N-ethy1-3-methylimidazolium acetate (C(2)minOAc) was applied. The SSF process was applied for a period of three days. As a result, 3.7 g ethanol/L (corresponding to a yield of 0.19 g of ethanol/g of glucose or 38.0 wt-% of the theoretical maximum) was obtained at 72 h. When fresh Lenga and Eucalyptus residues were fermented without any pretreatment, the SSF process yielded 0.017 and 0.002 g of ethanol/g of glucose, respectively (3.33 wt-% and 0.48 wt-% of the theoretical maximum, respectively). Thus, the pretreatment procedures resulted in a significant increase in ethanol production, therefore justifying the need of pretreatment prior to the co-enzyme hydrolysis and fermentation for this type of biomass. Further, the combination of IL pretreatment and use of SSF process demonstrated the high potential for bioethanol production from Lenga and Eucalyptus residues. Nevertheless, further improvement by optimization of operational conditions is required to maximize the ethanol yield. (C) 2015 Elsevier B.V. All rights reserved.
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