Developed ratiometric fluorescent probe as a logic platform for potential diagnosis of thyroid disease and diabetes and fluorescent ink

Iodine and glucose are indispensable substances whose abnormal levels are closely associated with a variety of diseases. Herein, CDs and RhB embedded Zr-metal-organic framework (RhB-CDs@MOF-808) as dual-emitting ratiometric fluorescent probe was fabricated via post-synthetic modification (PSM). The constructed ratiometric probe presents the emission at 450 nm of CDs was served as a reference to calibrate the emission at 575 nm attributed to RhB to achieve a remarkable self-calibration function. The fluorescence of probe is quenched by I-3(-) by virtue of inner filter effect (IFE), and the I-3(-) is reduced by glucose to achieve the recovery of fluorescence. In view of this, an analytical platform for the continuous detection of I-3(-) and glucose was established with wide linear ranges (I-3(-) : 0-220 mu M; glucose: 0-180 mu M) and low LODs (I-3(-) : 0.031 mu M; glucose: 0.026 mu M). The probe has been successfully exploited to the determination of iodine and glucose in real samples of food and biology. More interestingly, an INHIBIT logic gate was established to provides a new platform for the preliminary diagnosis of thyroid disease and diabetes in clinical medicine. The probe as fluorescent ink achieved the continuous detection of iodine and glucose on a test paper expanding its potential application in samples containing iodine and glucose. This is the unprecedented attempt to exploit a ratiometric fluorescent probe for continuous detection of iodine and glucose, which can provide a new approach for the development of low-cost, sensitive and credible biological and clinical diagnostic thyroid disease and diabetes.

Automated summary

The study developed a dual-emitting ratiometric fluorescent probe RhB-CDs@MOF-808 for continuous detection of iodine and glucose, with self-calibration function and low detection limits. The probe has been successfully applied in food and biological samples, providing a new platform for the preliminary diagnosis of thyroid disease and diabetes.