Firstly electrochemical examination of vildagliptin at disposable graphite sensor: Sensitive determination in drugs and human urine by square-wave voltammetry

The work describes the determination of vildagliptin at the pencil graphite electrode using cyclic voltammetry and square-wave voltammetry. Vildagliptin yielded a well-defined irreversible peak response in phosphate buffer (PBS 9.0) medium, at about +1.13 V by cyclic voltammetry. Using optimum square-wave voltammetry condi-tions, a good linear correlation was obtained between the anodic current signal and concentration, in the range of 2.94-49.98 mu M in PBS (pH 9.0) medium, the limit of detection and relative standard deviation were calculated as 82 nM (24.88 ng L-1) and 2.95%, respectively. The developed voltammetric method was successfully applied for vildagliptin determination without any separation in pharmaceutical preparations and urine samples.

Automated summary

This work successfully determined vildagliptin at a pencil graphite electrode using cyclic voltammetry and square-wave voltammetry. A well-defined irreversible peak response was obtained, showing a good linear correlation between anodic current signal and concentration of vildagliptin. The developed voltammetric method was successfully applied for determination in pharmaceutical preparations and urine samples without the need for separation.