期刊
MICROCHEMICAL JOURNAL
卷 170, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.microc.2021.106727
关键词
Raman; Probe; Spectroscopy; Periodontitis; Diabetes mellitus; Saliva
资金
- School of Dentistry, International Medical University [IMU 411/2018]
The study aimed to achieve chairside identification of specific biomarkers for diagnosing periodontal disease in diabetic patients at a very early stage using spectroscopic probe design and parameters. Analysis showed a significant association between the severity of chronic periodontitis and the rate of MMP-8/CTX release in diabetic patients.
The study was performed to have chairside identification of specific biomarkers for diagnosing periodontal disease in diabetic patients at a very early stage using spectroscopic probe design and parameters. Patients were divided into groups: Group (A) - healthy patients, Group (B) - patients with type 2 Diabetes Mellitus (DM) without chronic periodontitis, Group (C) - mild chronic periodontitis patients without type 2 DM, Group (D) moderate chronic periodontitis patients without type 2 DM, Group (E) - mild chronic periodontitis patients with type 2 DM, and Group (F) - moderate chronic periodontitis patients with type 2 DM. Conceptual and practical design for achieving chairside Raman probe has specific tips and optics applied based on temperature of the mouth. Activation of Matrix metalloproteinase-8 (MMP-8) within salivary specimens were analysed. For molecular docking studies, binding pocket was selected with larger SiteScore. MMP-8 and C-terminal cross-linked telopeptide (CTX) of type I collagen levels in saliva were analysed using MMP-8 Immunoassay. Raman measurements were carried out of salivary specimens. Continuous variables were compared by one-way analysis of variance. Rate of MMP-8/CTX release was significantly higher in diabetes chronic moderate periodontitis group (p < 0.05). Heat map colour scheme of green indicates increase while the yellow track lines indicate a decrease. Striking difference is seen in Amide regions amongst different groups with lowest intensities seen in diabetic chronic moderate groups and the highest amongst healthy patients (p < 0.05). Major peak at 1447 cm-1 differed in intensities between all groups. Chairside identification of specific biomarkers to diagnosing periodontal disease at a very early stage using spectroscopic probe design and parameters has shown potential.
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